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In this article, I briefly describe cloning vectors for higher plants. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning purposes is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken from a virus. … Read more >>
In this article, I briefly describe yeast cloning vectors. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning purposes is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken from a virus. The vector … Read more >>
In this article, I briefly describe the cloning vectors based on bacteriophages and cosmids. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning purposes is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken … Read more >>
In this article, I briefly describe the cloning vectors based on E.coli plasmids. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken from a … Read more >>
In this article, I briefly describe the methods of clone identification. Gene library The collection of different DNA sequences from an organism, which is the total genomic DNA of the organism, makes a gene library. Each DNA sequence is cloned into a vector for ease of purification, storage, and analysis. The first step in the … Read more >>
In this article, I briefly describe the making of a cDNA library, which starts with the synthesis of cDNA. Gene library The collection of different DNA sequences from an organism, which is the total genomic DNA of the organism, makes a gene library. Each DNA sequence is cloned into a vector for ease of purification, … Read more >>