Cloning vectors based on bacteriophages and cosmids

In this article, I briefly describe the cloning vectors based on bacteriophages and cosmids. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning purposes is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken … Read more >>

Cloning vectors based on E.coli plasmids

In this article, I briefly describe the cloning vectors based on E.coli plasmids. Cloning vectors A small piece of DNA into which a foreign DNA fragment is inserted for cloning purposes is known as a cloning vector. The cloning vector may be the plasmid from a bacterium, a higher organism’s cell, or DNA taken from … Read more >>

Methods of clone identification

In this article, I briefly describe the methods of clone identification. Gene library The collection of different DNA sequences from an organism, which is the total genomic DNA of the organism, makes a gene library. Each DNA sequence is cloned into a vector for ease of purification, storage, and analysis. The first step in the … Read more >>

Making of a cDNA library

In this article, I briefly describe the making of a cDNA library, which starts with the synthesis of cDNA. Gene library The collection of different DNA sequences from an organism, which is the total genomic DNA of the organism, makes a gene library. Each DNA sequence is cloned into a vector for ease of purification, … Read more >>

Antigen-antibody interaction- Complement fixation

In this article, I briefly describe complement fixation, which is an antigen-antibody interaction. Antigen-antibody interaction The antigen-antibody interaction is a bimolecular association. This does not lead to an irreversible chemical alteration in either the antibody or the antigen. This association involves many non-covalent interactions between the antigenic determinant (epitope) of the antigen and the variable-region … Read more >>

The antigen-antibody interaction- Immunofluorescence

In this article, I briefly describe the method of immunofluorescence, which is an antigen-antibody interaction. Antigen-antibody interaction The Interaction between antigen and antibody is a bimolecular association, which does not lead to an irreversible chemical alteration in either the antibody or the antigen. The antigen-antibody association involves many non-covalent interactions between the antigenic determinant (epitope) … Read more >>

Type-III Hypersensitivity

In this article, I briefly explain type-III hypersensitivity along with its effects. Hypersensitivity Hypersensitive reactions are the inflammatory reactions within the humoral or cell-mediated branches of the immune system. These reactions cause extensive tissue damage, or occasionally death. The reactions are immediate or may be delayed type depending on the symptoms. In 1963, P.G.H. Gell … Read more >>

The antigen-antibody interaction- Radioimmunoassay

In this article, I briefly describe the radioimmunoassay, which is a process of antigen-antibody interaction. Antigen-antibody interaction The Interaction between antigen and antibody is a bimolecular association, which does not lead to an irreversible chemical alteration in either the antibody or the antigen. The antigen-antibody association involves many non-covalent interactions between the antigenic determinant (epitope) … Read more >>

The antigen-antibody interaction- Enzyme-linked Immunosorbent Assay (ELISA)

In this article, I briefly describe the enzyme-linked immunosorbent assay, which is an antigen-antibody interaction. Antigen-antibody interaction The Interaction between antigen and antibody is a bimolecular association, which does not lead to an irreversible chemical alteration in either the antibody or the antigen. The antigen-antibody association involves many non-covalent interactions between the antigenic determinant (epitope) … Read more >>

Type-II hypersensitivity

In this article, I briefly describe type-II hypersensitivity with its effects. Hypersensitivity The inflammatory reactions within the humoral or cell-mediated branches of the immune system, causing extensive tissue damage or occasionally death, are known as hypersensitive reactions. The reactions are immediate or may be delayed type depending on the symptoms. In 1963, P.G.H. Gell and … Read more >>